Orientation selectivity, a fundamental feature observed throughout the early visual system, is first computed in the retina by orientation selective ganglion cells (OSGCs). Recent work has reported diverse mechanisms underlying On and Off OSGCs, yet less is known about the On-Off type. My preliminary calcium imaging data suggests that On-Off OSGCs are a major OSGC subtype in the mouse retina and they are sensitive to GABAA receptor antagonists. This proposal aims to determine the synaptic mechanisms underlying the orientation selectivity of On-Off OSGCs in the mouse retina and their central projection patterns. The lack of molecular markers for OSGCs has limited a mechanistic understand of orientation selectivity in the mammalian retina.
In Aim 1, we will perform calcium imaging of RGCs to screen for On-Off OSGCs and target them for single-cell recordings. Additionally, we will use pharmacology and ?-subunit specific manipulations of GABAA receptors on RGCs to determine the involvement of specific synapses in orientation selectivity.
In Aim 2, we will identify the projection patterns of On-Off OSGCs to dorsal LGN using viral retrograde tracers and transgenic labeling. Learning to perform whole-cell patch-clamp recordings and retrograde tracer injections are major training goals of this grant. Results from this work will yield a mechanistic understanding of retinal orientation selectivity and provide a link between this retinal feature selectivity and higher visual processing in the thalamocortical pathway.

Public Health Relevance

The retina diverges information from the visual scene into ~30 functionally distinct channels, each mediated by a subtype of retinal ganglion cells (RGCs). Understanding the circuit mechanisms involved in retinal visual feature selectivity and the organization of the axonal projections of RGC subtypes is necessary for a deeper understanding of visual system function in health and disease. Furthermore, the knowledge gained from the proposed study will have broader implications as it will address the general principles of sensory processing in the brain.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31EY029156-02
Application #
9894639
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Agarwal, Neeraj
Project Start
2019-04-01
Project End
2021-03-31
Budget Start
2020-04-01
Budget End
2021-03-31
Support Year
2
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Chicago
Department
Biology
Type
Schools of Medicine
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637