The hydrolysis and/or formation of phosphodiester bonds is of central importance in biology. Enzymes such as polymerases, recombinases, topoisomerases, reverse transcriptases, and others catalyze phosphoryl transfer reactions efficiently under physiological conditions. Studies of enzymological reaction mechanisms using biochemical techniques or synthetic models is currently an area of intensive interest. One extremely common but little studied enzyme mechanistic motif involves the use of two metal ions in the hydrolysis of phosphodiesters and other substrates. This proposal describes the development of selective catalysts for DNA hydrolysis that utilize this mechanistic motif. The compounds will be used to test hypotheses about enzyme mechanisms as well as for applications in biotechnology.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31GM018752-03
Application #
2838411
Study Section
Special Emphasis Panel (ZRG2-PSF (04))
Project Start
1998-12-01
Project End
Budget Start
1998-12-01
Budget End
1999-11-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
New York University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012