The proposed research will further our understanding of how a eukaryotic cell synthesizes ribosomes in an efficient and timely manner. Ribosome biogenesis is a complex process that is necessary for both cell maintenance and growth. NOP4 is an essential yeast gene that has been implicated in ribosome biogenesis. Specifically, it mediates pre-rRNA processing of the 60S ribosomal subunit, but its exact role in processing is unclear. Nop4p is a protein capable of binding RNA. Therefore, I propose to identify the specific molecules that interact with Nop4p in its role as an essential mediator of pre-rRNA processing. Methods such as the three-hybrid system and synthetic lethality will allow me to identify both RNA and protein molecules that interact with Nop4p. After identification of such molecules, I will be able to propose a model of Nop4p's function in ribosome biogenesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31GM019937-01
Application #
2803918
Study Section
Special Emphasis Panel (ZRG2-SSS-I (03))
Project Start
1999-02-06
Project End
Budget Start
1998-12-01
Budget End
1999-11-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Carnegie-Mellon University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Miles, Tiffany D; Jakovljevic, Jelena; Horsey, Edward W et al. (2005) Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol Cell Biol 25:10419-32
Horsey, Edward W; Jakovljevic, Jelena; Miles, Tiffany D et al. (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10:813-27