State-of-the-art dyes for DNA sequencing involve through-space (Forster) energy transfer from a donor fragment that absorbs the incident light, to an acceptor part that fluoresces at a longer wavelength. However, the resolution and sensitivities possible in such approaches are constrained by the physics of the system. The applicant's research is to investigate if another strategy for energy transfer can be used to produce dyes for DNA sequencing that are superior to any that are currently known. To do this, systems designed to facilitate through-bond energy transfer are being developed. Transfer of energy through bonds seems not to be constrained by the same parameters that limit Forster-based systems. The molecular characteristics that favor through-bond energy transfer are not widely understood. Consequently, parallel solid phase syntheses methods are being developed to prepare focused libraries of through-bond energy transfer cassettes that can be screened for desirable properties for sequencing applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31GM073571-02
Application #
7125024
Study Section
Special Emphasis Panel (ZRG1-F08 (29))
Program Officer
Toliver, Adolphus
Project Start
2005-09-01
Project End
2007-03-31
Budget Start
2006-09-01
Budget End
2007-03-31
Support Year
2
Fiscal Year
2006
Total Cost
$18,918
Indirect Cost
Name
Texas A&M University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
078592789
City
College Station
State
TX
Country
United States
Zip Code
77845