Phosphorylation events regulate cell function in a multiplicity of pathways. Despite the effort to understand the molecular mechanisms that govern sperm function, there is still very little known. Recently, a family of testis specific serine/threonine kinases was described. However, the characterization of this family, their activation and substrates as well as their specific function remains to be discovered. We have found evidence of these kinases in mature sperm both in mice and in humans. We have also found an interesting conservation pattern that dates back to invertebrates keeping the testis specificity. Because of these findings we believe that this family of kinases plays an important role in spermatogenesis and/or sperm function. In this proposal we propose to further describe and attempt to characterize this family of kinases focusing our effort in the mammalian members and utilizing the mouse model with a variety of biochemical, molecular biology and cell biology techniques. ? ? ?

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31HD049324-03
Application #
7495977
Study Section
Special Emphasis Panel (ZRG1-IMM-L (29))
Program Officer
Taymans, Susan
Project Start
2006-07-18
Project End
2010-07-17
Budget Start
2008-07-18
Budget End
2009-07-17
Support Year
3
Fiscal Year
2008
Total Cost
$28,273
Indirect Cost
Name
University of Massachusetts Amherst
Department
Veterinary Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
153926712
City
Amherst
State
MA
Country
United States
Zip Code
01003
Li, Yahui; Sosnik, Julian; Brassard, Laura et al. (2011) Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis. Mol Hum Reprod 17:42-56
Sosnik, Julian; Buffone, Mariano G; Visconti, Pablo E (2010) Analysis of CAPZA3 localization reveals temporally discrete events during the acrosome reaction. J Cell Physiol 224:575-80
Sosnik, Julian; Miranda, Patricia V; Spiridonov, Nikolay A et al. (2009) Tssk6 is required for Izumo relocalization and gamete fusion in the mouse. J Cell Sci 122:2741-9