The long term goal of this application is to determine how glucocorticoids regulate the expression of the pro-apoptotic gene Bnip3 in the developing rat brain. Central nervous system development uses apoptosis as a normal developmental process to eliminate excess cells. Bnip3, a pro-apoptotic gene, is highly expressed within the developing rat cortex and is upregulated by the synthetic glucocorticoid, dexamethasone. Clinical studies have shown several lines of research, which suggest that glucocorticoid regulation of Bnip3 may play a role in neonatal brain pathology. We anticipate the proposed experiments will provide pre-clinical evidence for some mechanisms involved in glucocorticoid endangerment of developing neurons. Upon completion of the proposed experiments we will determine if glucocorticoid treatment alters developmental gene expression of Bnip3 mRNA and protein in the rat cortex. Also, we will determine if glucocorticoids act directly on cortical neurons to regulate Bnip3 expression and finally we will determine if the Bnip3 promoter is directly regulated by glucocorticoid receptors.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31NS046959-02
Application #
6784077
Study Section
Special Emphasis Panel (ZRG1-F03A (20))
Program Officer
Leblanc, Gabrielle G
Project Start
2003-07-01
Project End
2006-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
2
Fiscal Year
2004
Total Cost
$35,222
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
785979618
City
Fort Collins
State
CO
Country
United States
Zip Code
80523