This proposal focuses on studying RNA silencing using an Agrobacterium-mediated transient assay system. RNA silencing was recently identified as an adaptive, antiviral host defense response in plants. RNA silencing limits the extent of a virus infection through sequence-specific cleavage of viral RNA. RNA silencing in plants is closely related to the process of RNA interference in animals, and quelling in fungi. Through genetic and biochemical analysis, the molecular basis for RNA silencing is partially understood. A key component of the RNA silencing pathway is the host RdRp. The RdRp is proposed to be important for the initiation and perhaps maintenance of the RNA silencing signal. A series of experiments to test the role of the RdRp in the two RNA silencing induction pathways are proposed. Many viruses encode RNA silencing suppressors. P1/HC-Pro, a silencing suppressor of potyviruses, will be used in the transient assay to further test its role in silencing suppression. Introducing a combination of components and effectors of the silencing pathway will allow for a more detailed understanding of the initiation, maintenance, and signaling stages of RNA silencing.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32AI051097-02
Application #
6622366
Study Section
Experimental Virology Study Section (EVR)
Program Officer
Greenfield, Teri L
Project Start
2002-04-01
Project End
Budget Start
2003-04-01
Budget End
2004-03-31
Support Year
2
Fiscal Year
2003
Total Cost
$48,148
Indirect Cost
Name
Oregon State University
Department
Other Basic Sciences
Type
Schools of Arts and Sciences
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339