Abstract

Leptospirosis is presumed to be the most geographically widespread zoonotic disease in the world, and regarded as a re-emerging infectious disease that effects human populations on a global scale. A severe pulmonary form of leptospirosis (SPFL) is appearing and results in high mortality among patients. The first successful isolation of leptospires from patients suffering from SPFL occurred in 1998 at the National Leptospirosis Reference Laboratory in Brazil. The research program to be undertaken during the period of training is based on the study of these isolates obtained from patients suffering from SPFL. More specifically, 1) the tools of proteomics will be used to identify and characterize proteins of the outer membrane of SPFL isolates and 2) characterization of an animal model of disease (guinea pig) which will be used to 3) identify leptospiral proteins expressed during infection. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32AI055235-02
Application #
6735702
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Schaefer, Michael R
Project Start
2003-04-01
Project End
2005-03-31
Budget Start
2004-04-01
Budget End
2005-03-31
Support Year
2
Fiscal Year
2004
Total Cost
$47,296
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Nally, Jarlath E; Whitelegge, Julian P; Bassilian, Sara et al. (2007) Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Infect Immun 75:766-73
Nally, Jarlath E; Chow, Emilie; Fishbein, Michael C et al. (2005) Changes in lipopolysaccharide O antigen distinguish acute versus chronic Leptospira interrogans infections. Infect Immun 73:3251-60
Nally, Jarlath E; Whitelegge, Julian P; Aguilera, Rodrigo et al. (2005) Purification and proteomic analysis of outer membrane vesicles from a clinical isolate of Leptospira interrogans serovar Copenhageni. Proteomics 5:144-52
Nally, Jarlath E; Fishbein, Michael C; Blanco, David R et al. (2005) Lethal infection of C3H/HeJ and C3H/SCID mice with an isolate of Leptospira interrogans serovar copenhageni. Infect Immun 73:7014-7
Nally, Jarlath E; Chantranuwat, Chavit; Wu, Xiao-Yang et al. (2004) Alveolar septal deposition of immunoglobulin and complement parallels pulmonary hemorrhage in a guinea pig model of severe pulmonary leptospirosis. Am J Pathol 164:1115-27
Bharti, Ajay R; Nally, Jarlath E; Ricaldi, Jessica N et al. (2003) Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3:757-71