Environmental stress has.played an enormous role in the evolution and physiology of organisms over time. Microbial ecosystems in particular have responded to environmental stress by developing complex biofilm community structures and novel gene products.
The aim of this proposal is to explore the role of oxidative stress in nature by examining global regulatory mechanisms in microbial mat communities using newly developed genetic techniques. More specifically, I propose to: 1) determine exogenous and endogenous levels of reactive oxygen species in cultured cyanobacterium and in field collected microbial mat samples; 2) determine the effects of reactive oxygen species on the growth of the cultured cyanobacterium Synechocystis sp 6803, and in situ with field collected microbial mat samples; and 3) identify biofilm associated genes regulated by oxidative stress using a DNA microarray designed to the cyanobacterium Synechocystis sp 6803. Taken together these studies would represent the first study to examine the global gene regulatory response of microbial mat biofilms exposed to oxidative stress in situ. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32AI056967-01X1
Application #
6790900
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Korpela, Jukka K
Project Start
2003-02-14
Project End
Budget Start
2003-02-14
Budget End
Support Year
1
Fiscal Year
2003
Total Cost
$4,400
Indirect Cost
Name
Nasa - Ames Research Center
Department
Type
DUNS #
009231648
City
Moffett Field
State
CA
Country
United States
Zip Code
94035
Foster, Jamie S; Singh, Abhay K; Rothschild, Lynn J et al. (2007) Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Arch Microbiol 187:265-79