The CDC2-related protein kinase, CDC2L5, shares extensive sequence homology and characteristics with CDK9, the kinase subunit of the general transcription elongation factor P-TEFb, which is also important for the replication of HIV in its host. This study will try to determine the functions of CDC2L5, in relation to CDK9's ability to activate general and HIV transcription. The kinase and transcription activities of CDC2L5 will be characterized and compared to those of CDK9. Moreover, since an RS domain common to all splicing factors is also found in CDC2L5, splicing assay will be performed to examine the possible involvement of CDC2L5 in HIV mRNA splicing. Finally, the expression of endogenous CDC2L5 will be suppressed by RNA interfering technique, followed by gene expression analysis with DNA microarray technology to determine the affected genes and thus the unique physiological function of CDC2L5. The information gains from this study could be useful for developing new methods for AIDS therapy by specifically targeting these cellular events governed by CDC2L5.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32AI058400-03
Application #
7023800
Study Section
AIDS Molecular and Cellular Biology Study Section (AMCB)
Program Officer
Gupta, Kailash C
Project Start
2004-03-01
Project End
2007-02-28
Budget Start
2006-03-01
Budget End
2007-02-28
Support Year
3
Fiscal Year
2006
Total Cost
$50,428
Indirect Cost
Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704