EBV is a ubiquitous herpesvirus that causes transformation of lymphocytes and leads to a variety of neoplasms. Immunosuppressed individuals often have active EBV disease that results in the establishment of lymphomas. I propose that immunosuppressive drugs lead to downregulation of T cell function and immune dysregulation resulting in increased EBV growth and transformation of B cells. My primary objective will be to establish and characterize lymphoblastoid cell lines spontaneously derived from patients with detectable EBV infection. These cell lines are an authentic model of EBV infection because the EBV-transformation occurred in vivo. I will use these cell lines to evaluate the role of immunosuppressants in contributing to the development of EBV related lymphoproliferative disorders. I will measure Fas/Fas ligand expression and the potential for induction of apoptosis. In addition, I will characterize the role of bcl-2 proteins regulating cell death in these EBV transformed lines. These experimental cell lines can provide direct benefit for the patients from which they were derived and will be crucial in preparing potential prophylactic mechanisms for preventing EBV related disease in immunosuppressed patient populations.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32CA071128-03
Application #
2712795
Study Section
Special Emphasis Panel (ZRG2-IVP (01))
Program Officer
Lohrey, Nancy
Project Start
1998-06-01
Project End
Budget Start
1998-06-01
Budget End
1999-05-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Stanford University
Department
Surgery
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305