The long-range objective of this project is to determine the functional significance of the multiple heparin sulfate chains on proteoglycans. Each chain may contribute to the optimal activity of the molecule. Alternatively, each chain may be required for a distinct function. The heparin sulfate chains of syndecan-1 are biologically active in mediating cell:substratum and cell:cell adhesion and in inhibiting cell invasion. To test the hypothesis that all three of the highly conserved heparin sulfate attachment sites are required for maximal biological activity, in specific aim 1, a panel of syndecan- 1 glycosaminoglycan deletion mutants will be prepared using oligonucleotide-directed mutagenesis.
In specific aim 2, myeloma cells transfected with the various mutated syndecan-1 cDNAs will be examined in functional assays to determine how alterations in glycosaminoglycan composition affect: (i) cell:substratum adhesion.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32CA071145-02
Application #
2517726
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1997-09-01
Project End
Budget Start
1997-09-01
Budget End
1998-08-31
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Arkansas for Medical Sciences
Department
Pathology
Type
Schools of Medicine
DUNS #
City
Little Rock
State
AR
Country
United States
Zip Code
72205