: Kaposi's sarcoma is the most commonly occurring cancer in persons infected with human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome (AIDS). Recent studies have provided strong evidence that Kaposi's sarcoma herpesvirus (KSHV or HHV-8) is the causative agent of Kaposi's sarcoma. To date, little is known about the detailed molecular biology of KSHV replication and pathogenesis. The purpose of the studies in this proposal is to understand the function of KSHV T1.1 RNA. Based on the hypothesis that T1.1 RNA is important for KSHV replication, it is important to know if it affects vira1 gene expression. It is also important to determine if T1.1 RNA directly interacts with viral or host cellular proteins. To address these questions, chemical modification mapping studies of the T1.1 RNA will determine accessible regions and identify on potential RNA protein binding motifs. This information will be used to design hammerhead ribozymes or anti-sense oligonucleotides that efficiently inhibit T1.1 RNA function. Affinity purification methods based on the T1.1 RNA mapping patterns will be used to identify cellular or viral proteins that form the T1.1 ribonucleoprotein complex.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32CA088516-01A1
Application #
6339923
Study Section
Special Emphasis Panel (ZRG1-AARR-4 (01))
Program Officer
Lohrey, Nancy
Project Start
2001-05-01
Project End
Budget Start
2001-05-01
Budget End
2002-04-30
Support Year
1
Fiscal Year
2001
Total Cost
$34,832
Indirect Cost
Name
University of Colorado Denver
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045