During the DNA damage response, mammalian RNAP II transcriptional activity is tightly regulated. Much of this regulation has been linked to modifications in the repetitive CTD of the largest subunit of RNAP II. CTD tyrosine phosphorylation increases following DNA damage and is phosphorylated by the proto-oncogene c- Abl. The overall goal of this proposal is to understand the role of tyrosine phosphorylation in the DNA damage response. Defects in the DNA damage response pathway lead to diseases and DNA damage induced apoptosis is the key to chemotherapy and radiation treatment.
The specific aims of this proposal include: identifying CTD interacting factors that are regulated by tyrosine phosphorylated CTD through a pull down assay; characterizing these proteins through in vitro and in vivo binding studies; determining the physiological significance of this regulation through various functional assays.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32CA110646-01A1
Application #
6937908
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Lohrey, Nancy
Project Start
2005-07-01
Project End
2008-06-30
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
1
Fiscal Year
2005
Total Cost
$49,928
Indirect Cost
Name
University of California San Diego
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093