The proposed work will study the modulation of ion channels in olfactory receptor neuron (ORN)s in response to odors. This work will be done using olfactory slices, a new tissue preparation. Various configurations of the patch-clamp technique will be employed to address the following specific aims: 1. Determine the properties of ORNs in the slice preparation. Using whole-cell and on-cell recording techniques the spontaneous activity will be observed and the voltage-dependent currents of ORNs examined. By filling the ORNs with Lucifier yellow the cell morphology will be correlated with the physiological measurements. 2. Evaluate to chemosensitivity of ORNs in the slice. Responses to odorants will be determined using the resistive whole-cell configuration and the conductances underlying the responses will be elucidated. Observe whether neighboring ORNs have similar chemosensitivity. 2. Describe the single channel properties of each channel that is modulated by a cocktail of odors. For each channel modulated by odors, determine if modulation by a second messenger can mimic the effect of the odor.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32DC000176-03
Application #
2391059
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1997-04-01
Project End
Budget Start
1997-04-01
Budget End
1997-11-14
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Boston University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
Ison, James R; Allen, Paul D; Oertel, Donata (2017) Deleting the HCN1 Subunit of Hyperpolarization-Activated Ion Channels in Mice Impairs Acoustic Startle Reflexes, Gap Detection, and Spatial Localization. J Assoc Res Otolaryngol 18:427-440
Oertel, Donata; Cao, Xiao-Jie; Ison, James R et al. (2017) Cellular Computations Underlying Detection of Gaps in Sounds and Lateralizing Sound Sources. Trends Neurosci 40:613-624
Cao, Xiao-Jie; Oertel, Donata (2017) Genetic perturbations suggest a role of the resting potential in regulating the expression of the ion channels of the KCNA and HCN families in octopus cells of the ventral cochlear nucleus. Hear Res 345:57-68
McGinley, Matthew J; Liberman, M Charles; Bal, Ramazan et al. (2012) Generating synchrony from the asynchronous: compensation for cochlear traveling wave delays by the dendrites of individual brainstem neurons. J Neurosci 32:9301-11
Golding, Nace L; Oertel, Donata (2012) Synaptic integration in dendrites: exceptional need for speed. J Physiol 590:5563-9
Oertel, Donata; Wright, Samantha; Cao, Xiao-Jie et al. (2011) The multiple functions of T stellate/multipolar/chopper cells in the ventral cochlear nucleus. Hear Res 276:61-9
Eisthen, H L; Delay, R J; Wirsig-Wiechmann, C R et al. (2000) Neuromodulatory effects of gonadotropin releasing hormone on olfactory receptor neurons. J Neurosci 20:3947-55