EKLF is a erythroid cell-specific transcription factor which is required for the production of Beta-globin. Regulation of EKLF by phosphorylation will be explored by: 1. Mapping its phosphorylation sites by the combination of HPLC, mass spectrometry and chemical sequencing. 2. Testing the effects of phosphorylation on its molecular properties by monitoring the effects of phosphorylation mutants on either DNA binding affinity or transcriptional activity. 3. Testing the effects of phosphorylation on its biological function by comparing the abilities of wild type or phosphorylation mutant EKLFs to rescue Beta-globin transcription in embryoid bodies that have been differentiated in vitro from EKLF -/- embryonic stem cells. These studies will provide a basis for us to identify protein kinnases which can phosphorylate EKLF and will contribute to the understanding of the mechanisms of both normal erythroid cell gene regulation during development and potential mechanisms for its disruption in leukemia.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32DK009772-02
Application #
6124744
Study Section
Special Emphasis Panel (ZRG4-HEM-2 (01))
Program Officer
Bishop, Terry Rogers
Project Start
1999-12-01
Project End
Budget Start
1999-12-01
Budget End
2000-11-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Mount Sinai School of Medicine
Department
Biochemistry
Type
Schools of Medicine
DUNS #
114400633
City
New York
State
NY
Country
United States
Zip Code
10029