The main objective of this proposal is to characterize the mechanism of insulin gene transcription in pancreatic beta cells. In particular, I want to define the role of ERK (extracellular signal-regulated kinase) in this process.
The specific aims of the project are: 1) to identify and characterize direct and indirect substrates of activated ERK that are linked to insulin gene transcription; 2) to determine the localization of ERK activation of specific substrates and transcription factors involved in insulin gene transcription; and 3) to characterize the effects of various stimuli (glucose, free fatty acids, insulin, cAMP). Ultimately, these studies will result in a better understanding of beta cell function and insulin signaling in relation to diabetes mellitus.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32DK010175-01
Application #
6298564
Study Section
Endocrinology Study Section (END)
Program Officer
Hyde, James F
Project Start
2001-07-01
Project End
Budget Start
2001-07-01
Budget End
2002-06-30
Support Year
1
Fiscal Year
2001
Total Cost
$34,832
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Pharmacology
Type
Schools of Medicine
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390
Ferrer, Jennifer R; Chokechanachaisakul, Attasit; Wertheim, Jason A (2015) New Tools in Experimental Cellular Therapy for the Treatment of Liver Diseases. Curr Transplant Rep 2:202-210
Uzarski, Joseph S; Su, Jimmy; Xie, Yan et al. (2015) Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development. J Vis Exp :e53271
Uzarski, Joseph S; Bijonowski, Brent M; Wang, Bo et al. (2015) Dual-Purpose Bioreactors to Monitor Noninvasive Physical and Biochemical Markers of Kidney and Liver Scaffold Recellularization. Tissue Eng Part C Methods 21:1032-43