The proposed experiments are designed to determine the structures of the protein complexes at the membrane interface in G protein signaling systems. The well-characterized vertebrate rod vision transduction pathway will be used as a model. By tethering specialized probes at specific sites on the surface of the G protein transducin, fluorescence resonance energy transfer and electron paramagnetic resonance spectroscopy will be utilized to determine the relative distance to the membrane. These distances will be tested for the inactive conformation of the protein, as well as with the activated conformation, and in the presence of downstream effectors.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32EY015048-01A1
Application #
6791876
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Mariani, Andrew P
Project Start
2004-07-01
Project End
2006-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
1
Fiscal Year
2004
Total Cost
$42,976
Indirect Cost
Name
Baylor College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Gross, Alecia K; Decker, Glenn; Chan, Fung et al. (2006) Defective development of photoreceptor membranes in a mouse model of recessive retinal degeneration. Vision Res 46:4510-8
Wensel, Theodore G; Gross, Alecia K; Chan, Fung et al. (2005) Rhodopsin-EGFP knock-ins for imaging quantal gene alterations. Vision Res 45:3445-53