Mutational Analysis of A Type Iis Restriction Enzyme

Waugh, David S.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Postdoctoral Individual National Research Service Award (F32)
• Project #: 5F32GM013909-02
• Application #: 3045279
• Study Section: Biomedical Sciences Study Section (BIOM)
• Project Start: 1991-03-15
• Budget Start: 1991-03-15
• Budget End: 1991-11-30
• Support Year: 2
• Fiscal Year: 1991

Institution

• Name: Massachusetts Institute of Technology
• Type: Schools of Arts and Sciences
• City: Cambridge
• State: MA
• Country: United States
• Zip Code: 02139

Publications

Waugh, D S; Sauer, R T (1994) A novel class of FokI restriction endonuclease mutants that cleave hemi-methylated substrates. J Biol Chem 269:12298-303

Waugh, D S; Sauer, R T (1993) Single amino acid substitutions uncouple the DNA binding and strand scission activities of Fok I endonuclease. Proc Natl Acad Sci U S A 90:9596-600