Udp-Galactose 4-Epimerase - Xray Crystallographic Studies

Thoden, James B.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Postdoctoral Individual National Research Service Award (F32)
• Project #: 5F32GM015950-02
• Application #: 2170447
• Study Section: Biomedical Sciences Study Section (BIOM)
• Project Start: 1994-10-01
• Budget Start: 1994-10-01
• Budget End: 1995-04-30
• Support Year: 2
• Fiscal Year: 1994

Institution

• Name: University of Wisconsin Madison
• Department: Biochemistry
• Type: Other Domestic Higher Education
• DUNS #: 161202122
• City: Madison
• State: WI
• Country: United States
• Zip Code: 53715

Publications

Thoden, J B; Gulick, A M; Holden, H M (1997) Molecular structures of the S124A, S124T, and S124V site-directed mutants of UDP-galactose 4-epimerase from Escherichia coli. Biochemistry 36:10685-95

Liu, Y; Thoden, J B; Kim, J et al. (1997) Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coli. Biochemistry 36:10675-84

Thoden, J B; Frey, P A; Holden, H M (1996) Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli. Biochemistry 35:2557-66

Thoden, J B; Frey, P A; Holden, H M (1996) Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism. Biochemistry 35:5137-44

Thoden, J B; Frey, P A; Holden, H M (1996) High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol. Protein Sci 5:2149-61

Lee, J K; Kaplan, S (1992) cis-acting regulatory elements involved in oxygen and light control of puc operon transcription in Rhodobacter sphaeroides. J Bacteriol 174:1146-57