The eukaryotic cell is highly organized, containing organelles that efficiently divide its many tasks. Membranes separate the organelles from one another, sustaining each in a unique chemical environment. In addition, proteinaceous channels exist in the membranes that recognize and transfer certain molecules between the cytosol and a specific organelle. Among the least understood of these channels are those that import proteins bound for certain organelles. The proposed research will use a combination of biochemistry and electrophysiology to identify and characterize the mechanism of proteins composing the outer membrane channel of a model organelle, the chloroplast. The long term objectives of the research are twofold: first, to understand on a molecular level how these channels form a semipermeable barrier between the organelle, and second, to determine how specific recognition of a signal sequence opens a channel.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM018720-01
Application #
2021499
Study Section
Biological Sciences 2 (BIOL)
Project Start
1997-07-14
Project End
Budget Start
1997-03-01
Budget End
1998-02-28
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Anatomy/Cell Biology
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065