We will investigate regulatory pathways in E. coli by monitoring global gene expression on microarrays. In particular, we will study nitrogen regulatory pathways and their connection to osmoregulatory pathways through the common intermediate glutamate. We will create microarrays of E. coli genes and develop methods for fluorescent labeling of E. coli cDNAs. As a first exercise, we will use microarrays to determine which genes are under regulatory control of the nitrogen regulatory C (NtrC ) protein in E. coli. Then we will investigate expression levels of all genes under nitrogen limiting conditions to determine how nitrogen limitation propagates downstream of a primary glutamine limitation to slow the growth rate of E. coli. Finally, we will investigate the roles of glutamate and K+ in cellular osmoregulation and the dual role of glutamate in osmoregulation and biosynthesis. Our development of a system for gene expression monitoring in bacteria will facilitate global expression analyses in bacteria.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM019862-01
Application #
2774204
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1999-07-08
Project End
Budget Start
1999-02-01
Budget End
2000-01-31
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Other Basic Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704