Gene expression in eukaryotes requires the exchanges of macromolecules between the cytoplasm and nucleus. Changes in growth, developmental state, cell cycle and viral infection all affect the type of molecules exchanged in a cell. Nuclear pore complexes (NPCs), spanning both sides of the fused nuclear membrane, are the structures through which all movement occurs. Therefore, understanding NPC structure and assembly is critical in understanding nuclear/cytoplasmic communication and is the long-term objective of this research. Toward this end, a fluorescence- based genetic strategy will be employed in yeast to identify factors required for NPC assembly. The role of identified factors in the assembly process will be elucidated using a variety of microscopy studies and biochemical methods. Finally, biochemical and genetically interacting factors will be identified. Together, these experiments will address the fundamental questions regarding the biogenesis and assembly of the NPC.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020308-03
Application #
6498540
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Flicker, Paula F
Project Start
2000-02-01
Project End
Budget Start
2002-02-01
Budget End
2002-06-30
Support Year
3
Fiscal Year
2002
Total Cost
$18,489
Indirect Cost
Name
Washington University
Department
Physiology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130