Sindbis virus is a member of the Alphavirus family which contains a number of human and animal pathogens. The goal of the proposal is to obtain an understanding at the molecular level of the events involved in the initiation of Sindbis virus minus strand RNA synthesis. An in vitro replication system will be used to examine the minimum cis-acting requirements for Sindbis virus minus-strand RNA synthesis. Using small template RNAs representing the minimal template for minus strand RNA synthesis mutagenesis, template competition and crosslinking assays will be used to determine the requirements for template recognition. Biochemical analyses using inhibitors of RNA synthesis will be aimed at determining the stability of the initiating and elongating replicase complexes. Finally using various template RNAs the role of sequences other than the minimal promoter in minus strand synthesis will be determined. These studies will be extended to plus-strand RNA synthesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020451-03
Application #
6518848
Study Section
Virology Study Section (VR)
Program Officer
Tompkins, Laurie
Project Start
2000-04-01
Project End
Budget Start
2002-04-01
Budget End
2002-07-31
Support Year
3
Fiscal Year
2002
Total Cost
$16,314
Indirect Cost
Name
Washington University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130