Gene expression in eukaryotes is often controlled at the level of the initiation of translation. eIF3 plays a central role in this process. It is responsible for ribosome subunit dissociation, it stabilizes the initiator Met-tRNA on the small ribosomal subunit and it is required for mRNA binding to the ribosome. p33, an RNA-binding subunit of eIF3, has recently been cloned and partially characterized. p33 is part of the """"""""core"""""""" of the eIF3 complex and is an essential protein in yeast. The elucidation of the function of the p33 subunit, the function of eIF3, and the mechanism of translation initiation in yeast in a longer perspective will be sought in the research under this training period via these four specific aims: 1. Identify the physiological RNA target for p33 binding using SELEX. 2. Isolate high and low copy number extragenic suppressors of p33 mutants. 3. Use microarrays and kinetic RT-PCR to examine mRNA from polysome fractions in p33 mutants. . 4. Elucidate the structure/function of eIF3 by protein crosslinking and by forming partial complexes (e.g., p33, p39 and p93) and testing their activities in vitro.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020799-02
Application #
6518889
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Tompkins, Laurie
Project Start
2001-05-01
Project End
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
2
Fiscal Year
2002
Total Cost
$44,212
Indirect Cost
Name
University of California Davis
Department
Biochemistry
Type
Schools of Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618