? This project proposes to investigate the role of self-processing in the fungal protein, galactose oxidase. In the mature form, this enzyme possesses a modified tyrosine cofactor that plays a crucial role in the enzyme's ability to oxidize alcoholic substrates to their corresponding aldehydes. Biogenesis of the cofactor requires both copper and dioxygen and may be connected to another post-translational modification that results in cleavage of a 1.7 kDa pro-sequence from galactose oxidase. Crystal structures of galactose oxidase before and after self-processing reveal significant conformational changes that may influence the copper coordination sphere and its oxidation state. Dioxygen is proposed to act as an allosteric effector of pro-sequence cleavage, which in turn is responsible for a conformational change allowing biogenesis to occur. These suppositions can be tested by characterizing the products of self-processing and separately measuring the rates of both pro-sequence cleavage and cofactor biogenesis. Perturbations to self-processing through added reagents or mutagenesis will be used to identify potentially rate-determining steps and develop a comprehensive mechanism that links pro-sequence cleavage to cofactor biogenesis. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM065010-01A1
Application #
6583605
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Okita, Richard T
Project Start
2003-06-30
Project End
Budget Start
2003-01-01
Budget End
2003-12-31
Support Year
1
Fiscal Year
2003
Total Cost
$41,608
Indirect Cost
Name
University of California Berkeley
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Humphreys, Kristi J; Mirica, Liviu M; Wang, Yi et al. (2009) Galactose oxidase as a model for reactivity at a copper superoxide center. J Am Chem Soc 131:4657-63