This study asks what factors are involved in maintaining cell polarity during a series of unequal cell divisions in embryos of the roundworm C. elegans. Mutant embryos with prolonged cell cycles lose cell polarity and undergo abnormally equal cell divisions. I will reexamine cell division phenotypes in slow-cleaving mutants. Several mutant strains will be subjected to RNA-mediated interference (RNAi) to find out what additional genetic insults will modify their phenotype. Genes that affect kinetics of either cell cycle or polarity are expected to be recovered in this screen, and the RNiI method will allow genes of interest to be rapidly isolated for further analysis. At least two genes already known to affect both early cell cycle timing and later differentiation will also be cloned and functionally studied. This project aims to shed light on basic mechanisms of animal cells, in particular those modulating rates of DNA replication and cytoplasmic reorganization.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
3F32GM066546-03S1
Application #
6990298
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Portnoy, Matthew
Project Start
2002-08-15
Project End
2005-08-14
Budget Start
2004-08-15
Budget End
2005-08-14
Support Year
3
Fiscal Year
2005
Total Cost
$24,464
Indirect Cost
Name
University of Oregon
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
948117312
City
Eugene
State
OR
Country
United States
Zip Code
97403