Frameshift is relevant to both plant and mammalian viruses, including Human Immunodeficiency Virus Type 1 (HIV-1) and Human Coronavirus (HCV). This frameshift allows for the translation of the RNA dependent RNA polymerase (Rd-RP). BYDV is a well-characterized plant virus with several unique translational processes, including a long distance base pairing which allows -1 frameshift. Nucleotides on the bulge of the adjacent downstream stem loop (ADSL) base pair to the long distance frameshift element (LDFE), four kilobases downstream. This novel mechanism requires further characterization since other cis acting components both distal and proximal to the frameshift site were found to influence frameshift. These regions will be assessed through structural analysis, site directed mutagenesis and in vitro and in vivo frameshift assays within wheat and oat protoplast transfection systems to quantitatively measure frameshift efficiencies. These assays will delineate the responsible RNA domains, necessary for frameshift.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM067477-03
Application #
6894257
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Haynes, Susan R
Project Start
2003-06-23
Project End
2006-02-28
Budget Start
2005-06-23
Budget End
2006-02-28
Support Year
3
Fiscal Year
2005
Total Cost
$36,055
Indirect Cost
Name
Iowa State University
Department
Other Basic Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
005309844
City
Ames
State
IA
Country
United States
Zip Code
50011