The broad long-term objectives to this proposal are to identify new factors involved in histone mRNA 3' end formation both biochemically and genetically and to characterize those proteins in vivo and in vitro.
Specific Aim 1 is designed to use a combination of classical and innovative biochemical techniques to identify and purify proteins binding in a functionally relevant manner to histone pre-mRNA in HeLa nuclear extract.
Specific Aim 2 will involve the use of a novel GFP based genetic screen to be performed in Drosophila melanogaster aimed at identifying loss of function mutants in the process. This screen will be performed to near saturating levels with the aid of an embryo sorter.
Specific Aim 3 is to develop an in vivo system to study the function relevance of identified factors as well as to characterize them. This in vivo assay may also be applied in a novel siRNA-based genetic screen in mammalian cells. An in vitro system will also be developed to study histone mRNA 3' end formation in the context of active transcription. Histone mRNA 3' end formation is intimately related to the cell cycle and DNA replication, thus understanding this process may lead to the production of more effective chemotherapeutic agents.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM070101-03
Application #
7096570
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Haynes, Susan R
Project Start
2004-08-01
Project End
2007-07-31
Budget Start
2006-08-01
Budget End
2007-07-31
Support Year
3
Fiscal Year
2006
Total Cost
$50,428
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Biochemistry
Type
Schools of Medicine
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Sullivan, Kelly D; Mullen, Thomas E; Marzluff, William F et al. (2009) Knockdown of SLBP results in nuclear retention of histone mRNA. RNA 15:459-72
Cakmakci, Nihal G; Lerner, Rachel S; Wagner, Eric J et al. (2008) SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein. Mol Cell Biol 28:1182-94
Wagner, Eric J; Burch, Brandon D; Godfrey, Ashley C et al. (2007) A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processing. Mol Cell 28:692-9
Wagner, Eric J; Ospina, Jason K; Hu, Yue et al. (2006) Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein. RNA 12:1206-18
Wagner, Eric J; Marzluff, William F (2006) ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase. Mol Cell Biol 26:6702-12
Richardson, Richard T; Alekseev, Oleg M; Grossman, Gail et al. (2006) Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation. J Biol Chem 281:21526-34
Wagner, E J; Berkow, A; Marzluff, W F (2005) Expression of an RNAi-resistant SLBP restores proper S-phase progression. Biochem Soc Trans 33:471-3