The proposed research will study the dynamics of water in nanoscopic environments and water-protein interactions in nanoscopic environments using ultrafast multidimensional infrared stimulated vibrational echoes with full phase information. Such experiments provide vibrational echo correlation spectra, which are akin to multidimensional NMR techniques except that vibrational echo correlation spectroscopy directly examines the structural degrees of freedom of biological systems on time scales not accessible by other methods. The experiments and associated theory examine dynamics, structure and their interrelationship. The new work builds on the successful application of multidimensional vibrational echo methods by the Fayer research group. For decades, vibrational spectroscopy has been important in the study of biological systems. The application and development of vibrational echo correlation spectroscopy will enhance vibrational spectroscopy as a tool with an impact as great as the advance of magnetic resonance into multidimensional techniques.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM071162-02
Application #
6891418
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Flicker, Paula F
Project Start
2004-04-01
Project End
2007-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
2
Fiscal Year
2005
Total Cost
$43,976
Indirect Cost
Name
Stanford University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Finkelstein, Ilya J; Massari, Aaron M; Fayer, M D (2007) Viscosity-dependent protein dynamics. Biophys J 92:3652-62
Massari, Aaron M; McClain, Brian L; Finkelstein, Ilya J et al. (2006) Cytochrome c552 mutants: structure and dynamics at the active site probed by multidimensional NMR and vibration echo spectroscopy. J Phys Chem B 110:18803-10
Finkelstein, Ilya J; Goj, Anne; McClain, Brian L et al. (2005) Ultrafast dynamics of myoglobin without the distal histidine: stimulated vibrational echo experiments and molecular dynamics simulations. J Phys Chem B 109:16959-66
Massari, Aaron M; Finkelstein, Ilya J; McClain, Brian L et al. (2005) The influence of aqueous versus glassy solvents on protein dynamics: vibrational echo experiments and molecular dynamics simulations. J Am Chem Soc 127:14279-89