Large numbers of novel non-protein-coding RNAs (ncRNAs) have been characterized in worms, plants, insects, and mammals by experimental approaches. However, with the advent of large-scale genome sequencing, especially in humans and mice, literally hundreds of additional ncRNAs of unknown classification and function have been identified. In mice alone, the ncRNAs are thought to represent approximately 1/3 of all transcription units in the genome. ncRNA function remains poorly understood since past research has focused primarily on protein coding genes and the proteome. To begin to close the knowledge gap on these types of transcription units, firstly, bioinformatics-based filtering of mouse putative non coding mRNA-like RNAs identified by RIKEN-FANTOM will be conducted to identify novel candidates for functional studies Secondly, molecular expression analysis of a selected set of ncRNAs will be conducted to identify tissue specific and/or abundantly expressed transcripts. Following from the molecular analysis, a select group of non-coding RNAs will be analyzed by loss-of-function genetics in mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM071196-01
Application #
6793016
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Tompkins, Laurie
Project Start
2004-08-01
Project End
2006-07-31
Budget Start
2004-08-01
Budget End
2005-07-31
Support Year
1
Fiscal Year
2004
Total Cost
$41,796
Indirect Cost
Name
Sanger Institute
Department
Type
DUNS #
346013253
City
Cambridge
State
Country
United Kingdom
Zip Code