Chemotactic bacteria sense chemical gradients with high sensitivity over a wide concentration range. The goal of the proposed research is to elucidate the functional architecture of the chemoreceptor signaling complex of Escherichia coli. The complex is composed of transmembrane receptor proteins that operate in cooperative teams with a histidine kinase, CheA, and a coupling protein, CheW, to amplify chemical stimuli into cytoplasmic signals that control cell motility. To determine how CheW and CheA interact with the receptor signaling domains, I propose to isolate receptor mutants that are specifically defective in those interactions, using a series of genetic and biochemical tests to identify the desired mutants. The locations of CheA and CheW in receptor signaling teams will be investigated by using single-chain receptors - molecules with two covalently connected subunits - to control the subunit composition and geometry in higher-order receptor complexes. These studies should provide general knowledge of the molecular signaling strategies of chemoreceptors and possibly valuable new insights into virulence mechanisms, as well, because chemotaxis and chemoreceptors are associated with pathogenesis in many types of bacteria.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM072406-02
Application #
6927304
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Rodewald, Richard D
Project Start
2004-08-01
Project End
2007-07-31
Budget Start
2005-08-01
Budget End
2006-07-31
Support Year
2
Fiscal Year
2005
Total Cost
$43,976
Indirect Cost
Name
University of Utah
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Mowery, Patricia; Ames, Peter; Reiser, Rebecca H et al. (2015) Chemotactic Signaling by Single-Chain Chemoreceptors. PLoS One 10:e0145267
Mowery, Patricia; Ostler, Jeffery B; Parkinson, John S (2008) Different signaling roles of two conserved residues in the cytoplasmic hairpin tip of Tsr, the Escherichia coli serine chemoreceptor. J Bacteriol 190:8065-74