Retinoids are known to be important in a variety of processes during development. Previous studies have shown that both retinoid and FGF signaling are required for anteroposterior patterning.
The specific aims are: 1) to determine if ets domain transcription factors interact with RAR and FGF signaling pathways, 2) to show the necessity for RAR regulation of Xcad expression in early anteroposterior development and 3) to show RAR and Wnt signaling act synergistically to pattern the trunk and posterior. We will employ MO-mediated gene knockdown and microinjection of specific reagents to determine the function of RAR isoforms, Wnt signaling as well as interactions with ets domain transcription factors. Whole mount in situ hybridization, real time PCR, microinjections, and embryological manipulations will be employed to discern the effects of loss-of-function in certain genes and their affects on the function of RAR, Wnt and FGF signaling. The long-term goal of the proposed research is to understand the role of retinoid signaling during vertebrate axial patterning. The results should be widely applicable to the study of other vertebrate model systems.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM073473-02
Application #
7035810
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Haynes, Susan R
Project Start
2005-02-01
Project End
2007-01-31
Budget Start
2006-02-01
Budget End
2007-01-31
Support Year
2
Fiscal Year
2006
Total Cost
$50,428
Indirect Cost
Name
University of California Irvine
Department
Anatomy/Cell Biology
Type
Schools of Arts and Sciences
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Janesick, Amanda; Shiotsugu, Jason; Taketani, Mao et al. (2012) RIPPLY3 is a retinoic acid-inducible repressor required for setting the borders of the pre-placodal ectoderm. Development 139:1213-24