My goal is to understand the systematic modulation of gene expression in response to problems during DNA replication. Failure to complete DNA replication in a timely manner can lead to cell death or genome instability, often associated with cancer. In many organisms, the effects of DNA damage and other causes of distress during replication are often mitigated through transcriptional responses. Recently, a novel global transcriptional response was identified in a functional genomic analysis of Bacillus subtilis cells perturbed in DNA replication. At least 50 of these genes are thought to be regulated by DnaA, an essential and well conserved ATPase, transcription factor, and replication initiator. I plan to identify the genes directly regulated by DnaA in B. subtilis, address how DnaA is activated as a transcriptional regulator, and examine how its target promoters are structured to respond to it. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM076950-02
Application #
7159383
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Portnoy, Matthew
Project Start
2005-12-01
Project End
2008-11-30
Budget Start
2006-12-01
Budget End
2007-11-30
Support Year
2
Fiscal Year
2007
Total Cost
$48,796
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139