How do genes turn on and off? If DNA sequence remains unchanged, changes in transcription are caused by differences in DNA-associated factors. So understanding chromatin composition changes within particular regions of DNA is vital to understanding gene expression. This proposal aims to better understand the role of chromatin dynamics in gene expression by examining how ribosomal DNA (rDNA) chromatin composition relates to rDNA transcription in the yeast Saccharomyces cerevisiae using mass spectrometry. This project has three specific aims. The first is to purify native rDNA chromatin from yeast cells. Second, the identity [and locations] of bound proteins and nature of histone modifications will be determined using mass spectrometry. Third, differences in chromatin composition and histone modifications between actively transcribed and silent rDNA will be determined. Future directions include extending the technology from yeast to mammalian cells. Chromatin composition at crucial loci could be followed through developmental transudations, environmental changes, or during oncogenesis. My ultimate goal is the complete characterization of an organism's """"""""chromatome:"""""""" the identity, quantity, and location of all proteins associated with every DNA sequence and their modification states through development, aging and disease. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM076989-02
Application #
7274254
Study Section
Special Emphasis Panel (ZRG1-F04B-A (20))
Program Officer
Flicker, Paula F
Project Start
2006-09-01
Project End
2008-08-31
Budget Start
2007-09-01
Budget End
2008-08-31
Support Year
2
Fiscal Year
2007
Total Cost
$48,796
Indirect Cost
Name
Rockefeller University
Department
Miscellaneous
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Donlin, Laura T; Andresen, Christian; Just, Steffen et al. (2012) Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization. Genes Dev 26:114-9