The process of signal transduction and the genetic relationship of the genes involved as well as their targets have been intensely studied and we are beginning to get a clear idea of how these signals specify cell fates and direct development. Still poorly understood is the mechanism by which signals are translated into the activation and/or repression of downstream target genes. Studies on pattern formation in the leg of Drosophila have focused on two major signaling pathways that create a distalizing gradient in the formation of the Proximo-Distal (PD) axis. The current model suggests that this gradient is differentially read out by at least three target genes along the PD axis. This model is supported by genetic and molecular studies; however the mechanism by which this gradient is read remains unknown. The focus of this proposal is to test the hypothesis that these signals are in fact directly read out by the target genes and done so in a graded fashion. The approach taken will involve the isolation and dissection of cis-regulatory elements that drive expression of reporter genes in response to the gradient. These elements will be tested through targeted mutagenesis and binding assays to determine the factors responsible for transcriptional activation. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM077860-03
Application #
7414375
Study Section
Special Emphasis Panel (ZRG1-F05-J (20))
Program Officer
Portnoy, Matthew
Project Start
2006-05-01
Project End
2009-04-30
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
3
Fiscal Year
2008
Total Cost
$50,428
Indirect Cost
Name
Columbia University (N.Y.)
Department
Biochemistry
Type
Schools of Medicine
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032