Mutagenesis screens in zebrafish have been shown to be a powerful way to identify critical genes in pathways of vertebrate blood development. The mutagenesis screen proposed here will uncover genes whose mutation leads to abnormal granulocytic cell development. Careful analysis of the blood lineages in mutant fish will determine whether the developmental defects are confined to the granulocyte lineage or affect multiple blood cell types. Our hypothesis is that a subset of these mutant fish will exhibit blood cell abnormalities similar to Myelodysplastic syndrome (MDS). MDS is a devastating disease in children and adults, that can affect several myeloid blood cell types and often progresses to acute leukemia. MDS is associated with specific chromosomal deletions, suggesting that genes within these regions are responsible, for the disease which have yet to be identified

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HD041330-02
Application #
6526949
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Hewitt, Tyl
Project Start
2002-09-01
Project End
Budget Start
2002-09-01
Budget End
2003-08-31
Support Year
2
Fiscal Year
2002
Total Cost
$44,212
Indirect Cost
Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215
Liu, Ting Xi; Rhodes, Jennifer; Deng, Min et al. (2007) Dominant-interfering C/EBPalpha stimulates primitive erythropoiesis in zebrafish. Exp Hematol 35:230-9
Rhodes, Jennifer; Hagen, Andreas; Hsu, Karl et al. (2005) Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish. Dev Cell 8:97-108