The goal of this research project is to use the principles of hierarchically organized self-assembly to design a multiplexed sequence specific DNA detection strategy based on semiconductor quantum dots (QDs) with applications in genomic analysis. Water-soluble and stable semiconductor QDs will be selected, synthesized and modified to be used as a substitute for conventional fluorophores. Furthermore, procedures to efficiently attach different oligonucleotide recognition elements capable of selective binding to specific targets will be tested. An array of various types of QDs will be evaluated as a detection system, based on their ability to utilize the molecular recognition properties of the oligonucleotides to participate in a self-assembly process that can visualized via changes in spectroscopic characteristics of the QDs. The results of these proof-of concept studies can be used the implementation of truly interdisciplinary approaches to the analysis of genomic information.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HG000222-01
Application #
6310890
Study Section
Genome Study Section (GNM)
Program Officer
Graham, Bettie
Project Start
2000-11-01
Project End
Budget Start
2000-11-01
Budget End
2001-10-31
Support Year
1
Fiscal Year
2000
Total Cost
$30,916
Indirect Cost
Name
Northwestern University at Chicago
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Evanston
State
IL
Country
United States
Zip Code
60201
Ivanisevic, Albena; McCumber, Kim V; Mirkin, Chad A (2002) Site-directed exchange studies with combinatorial libraries of nanostructures. J Am Chem Soc 124:11997-2001
Ivanisevic, A; Im, J H; Lee, K B et al. (2001) Redox-controlled orthogonal assembly of charged nanostructures. J Am Chem Soc 123:12424-5
Ivanisevic, A; Mirkin, C A (2001) ""Dip-Pen"" nanolithography on semiconductor surfaces. J Am Chem Soc 123:7887-9
Fain, P R; Luty, J A; Guo, Z et al. (1991) Localization of the highly polymorphic microsatellite DXS456 on the genetic linkage map of the human X chromosome. Genomics 11:1155-7