Vascular endothelium responds to shear stress due to blood flow by releasing nitric oxide (NO). Deficiencies in this vasodilator has been implicated in diseases such as essential hypertension, coronary artery insufficiency and chronic heart failure. However, Because of the short T1/2 of this free radical (<6 Sec.) the mechanism by which shear stress induces NO release and the components of the shear stress activated pathway are unknown. We propose to investigate this mechanism using our unique published porphyrin/Nafion coated carbon fiber microsensor for on line detection of NO combined with fura-2 fluorescence and patchclamp techniques for monitoring Ca2+ flux. Shear stress elevates cytosolic Ca2+ ([Ca2+]i) which is necessary for the activation of Ca2+

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL009072-01A1
Application #
2213999
Study Section
Experimental Cardiovascular Sciences Study Section (ECS)
Project Start
1995-09-02
Project End
Budget Start
1995-06-01
Budget End
1996-05-30
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Duke University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705