My research proposal is aimed at studying the intracellular calcium release channels (ICRC) with reference to cardiac ryanodine receptor (RyR) i.e., ryanodine receptor 2. RyR2 is the major intracellular calcium release channel in the heart, it regulates intracellular calcium in response to extracellular calcium that enters via dihydropyridine receptors in the plasma membrane. This phenomenon is referred to as calcium release. RyR2 is also found in the neurons of the brain along with RyR1, RyR3 and inositol 1, 4, 5-tris phosphate receptors (IP3R). My studies are aimed essentially at the understanding of the molecular physiology of RyR2, its structure, time course expression and tissue distribution in heart and brain of mouse embryo along with RyR1, RyR3 and IP3R. The availability of immunological tools i.e., common and isoform-specific antibodies for each class of ICRC will enable me to carry out immunolocalization studies of ICRC. Heart disease is one of the leading causes of death. My studies aim at the understanding of the developmental and age-related changes which are associated with the ICRC in mammalian heart and brain.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL010298-01
Application #
6054950
Study Section
Special Emphasis Panel (ZRG1-HEM-2 (01))
Project Start
2000-04-01
Project End
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
1
Fiscal Year
2000
Total Cost
$46,300
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Jeyakumar, Loice H; Gleaves, Linda A; Ridley, Bettye D et al. (2002) The skeletal muscle ryanodine receptor isoform 1 is found at the intercalated discs in human and mouse hearts. J Muscle Res Cell Motil 23:285-92
Jeyakumar, L H; Ballester, L; Cheng, D S et al. (2001) FKBP binding characteristics of cardiac microsomes from diverse vertebrates. Biochem Biophys Res Commun 281:979-86