Abstract

Heart disease is one of the major cause of death in the world. Identifying the molecular events during heart development will permit the advancement of preventing and treatment heart disease. This project will focus on elucidating the role of MEF2D during heart development with particular attention to the differentiation of muscle cells. MEF2D conditional null mice will be generated and used to exam the role of MEF2D in muscle development. Concurrently, MEF2D inactivated embryonic stem (ES) cells will be employed to study the role of MEF2D during cell differentiation in vitro. Using over expressed MEF2D ES cells, we will perform microarray analysis to identify MEF2D's downstream targets. Biochemical experiments will be performed on these newly identified genes to determine the transcriptional pathways that MEF2D regulates during heart development.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL074663-01
Application #
6694986
Study Section
Special Emphasis Panel (ZRG1-F10 (20))
Program Officer
Commarato, Michael
Project Start
2003-07-16
Project End
2006-07-15
Budget Start
2003-07-16
Budget End
2004-07-15
Support Year
1
Fiscal Year
2003
Total Cost
$41,608
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Biochemistry
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Phan, Dillon; Rasmussen, Tara L; Nakagawa, Osamu et al. (2005) BOP, a regulator of right ventricular heart development, is a direct transcriptional target of MEF2C in the developing heart. Development 132:2669-78