The broad objective is to develop an analysis strategy for heart expression data and use it to identify without any preconceived knowledge of disease-associated genes the key genes, their targets, and their regulatory pathway, and to validate these results in biological systems.
Specific Aims are: I: Link hypertrophy associated genes to transcriptional regulatory networks. II: Optimize the strategy for application to other cardiac gene expression data sets. Health Relatedness: A better understanding of the function of a p300- driven network in vivo is essential to guiding drug development both in cancer and heart disease. The experimental design includes running microarrays on murine models of hypertrophy, generating and applying novel statistical approaches to microarray data to extract genes related to hypertrophy, generating mathematical representations to visualize global expression profiles in 3D, studying promoter organization in silico to identify complex regulatory elements, searching the eukaryotic promoter database for these complex elements to find co-regulated genes, validating the complex elements in vivo using ChIP assay, overlapping the network from microarrays and literature to promoter driven network to find unknown candidate genes. ? ?

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HL083673-02
Application #
7233688
Study Section
Special Emphasis Panel (ZRG1-DIG-B (20))
Program Officer
Meadows, Tawanna
Project Start
2006-04-08
Project End
2009-04-07
Budget Start
2007-04-08
Budget End
2008-04-07
Support Year
2
Fiscal Year
2007
Total Cost
$52,898
Indirect Cost
Name
University of Miami School of Medicine
Department
Pharmacology
Type
Schools of Medicine
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146
Shehadeh, Lina A; Sharma, Salil; Pessanha, Mônica et al. (2013) MicroRNA-20a constrains p300-driven myocardial angiogenic transcription by direct targeting of p300. PLoS One 8:e79133
Rodrigues, Claudia O; Shehadeh, Lina A; Hoosien, Michael et al. (2011) Heterogeneity in SDF-1 expression defines the vasculogenic potential of adult cardiac progenitor cells. PLoS One 6:e24013
Wilson, Amber; Shehadeh, Lina A; Yu, Hong et al. (2010) Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells. BMC Genomics 11:229
Shehadeh, Lina A; Yu, Kristine; Wang, Liyong et al. (2010) SRRM2, a potential blood biomarker revealing high alternative splicing in Parkinson's disease. PLoS One 5:e9104
Adi, Nikhil; Mash, Deborah C; Ali, Yousuf et al. (2010) Melatonin MT1 and MT2 receptor expression in Parkinson's disease. Med Sci Monit 16:BR61-7
Wu, Qiuling; Ma, Qi; Shehadeh, Lina A et al. (2010) Expression of the Argonaute protein PiwiL2 and piRNAs in adult mouse mesenchymal stem cells. Biochem Biophys Res Commun 396:915-20
Shehadeh, Lina; Mitsi, Georgia; Adi, Nikhil et al. (2009) Expression of Lewy body protein septin 4 in postmortem brain of Parkinson's disease and control subjects. Mov Disord 24:204-10
Garamszegi, Nandor; Garamszegi, Susanna P; Shehadeh, Lina A et al. (2009) Extracellular matrix-induced gene expression in human breast cancer cells. Mol Cancer Res 7:319-29