This proposal aims to examine the role of 5-HT1A receptor in modulating anxiety.
We aim to determine the brain region and developmental time point during which 5-HT1A receptors establish normal or """"""""wild-type"""""""" (WT) anxiety levels for the lifetime of the animal. The proposed studies stem from our findings using inducible 5-HTIA rescue (1AR) mice, which conditionally express 5-HTIA receptors only in certain postsynaptic sites. Our findings suggest that the expression of postsynaptic 5-HT1A receptors is required sometime between postnatal days 5 and 21 for mice to exhibit WT rather than 5-HT1A knockout (1AKO) (increased) anxiety levels in adulthood. We will use a combination of molecular biological and behavioral tools to test our hypotheses. We propose to further behaviorally characterize the existing 1AR mice to more narrowly define the critical time for the 5-HT1A receptor to establish WT anxiety levels. We also propose to generate multiple novel 1AR lines using the bacterial artificial chromosome (BAC) strategy to dissect more precisely where and when the lasting effect on anxiety levels, established by the 5-HTIA receptor, takes place. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32MH065791-03
Application #
6863687
Study Section
Special Emphasis Panel (ZRG1-F02A (20))
Program Officer
Curvey, Mary F
Project Start
2003-03-01
Project End
2005-04-30
Budget Start
2005-03-01
Budget End
2005-04-30
Support Year
3
Fiscal Year
2005
Total Cost
$10,258
Indirect Cost
Name
Columbia University (N.Y.)
Department
Neurosciences
Type
Schools of Medicine
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032
Dulawa, Stephanie C; Holick, Kerri A; Gundersen, Brigitta et al. (2004) Effects of chronic fluoxetine in animal models of anxiety and depression. Neuropsychopharmacology 29:1321-30