The proposed experiments focus on visualization of an quantitation of the changes in autophosphorylated and non-phosphorylated Calcium/calmodulin dependent protein kinase II (CaMKII) in hippocampal slices after induction of LTP or LTD. Induction of new synthesis of CaMKII by stimulation that induce LTP will be verified with inhibitors of protein synthesis, with phosphorothioate antisense oligonucleotide, or with adenovirus delivered antisense RNA. A better understanding of the role of CaMKII in synaptic plasticity and of possible """"""""metaplasticity"""""""" mechanism is expected. Such an understanding may lead to clues to improve learning and memory in people with diseases such as Alzheimer's disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32NS010660-01
Application #
2647498
Study Section
Special Emphasis Panel (ZRG1-NLS-1 (01))
Program Officer
Oliver, Eugene J
Project Start
1998-08-01
Project End
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125
Ouyang, Yannan; Wong, Michael; Capani, Francisco et al. (2005) Transient decrease in F-actin may be necessary for translocation of proteins into dendritic spines. Eur J Neurosci 22:2995-3005