This application is for a 4-year International Research Scientist Development Award for the study of anti- bacterial immunity and vaccine development. The candidate will be under the mentorship of Dr. Stephen Calderwood and Dr. Edward Ryan at the Massachusetts General Hospital, Boston, Massachusetts, and Dr. Firdausi Qadri, at the ICDDR.B: Centre for Health and Population Research, Dhaka, Bangladesh (ICDDR.B). The candidate is currently completing a fellowship in Pediatric Infectious Diseases and plans to pursue an academic career studying enteric diseases of importance in developing countries. In the proposed program, the trainee will spend at least 50% of his time at the ICDDR.B. The training program will include courses and symposia focused on bacterial genetics, immunology and vaccine development. Hands-on experience will broaden the trainee's skills in the laboratory study of adaptive immunity, in the conduct and analysis of large observational studies in human subjects, and in the laboratory techniques necessary for translational research in vaccine development. The proposal is focused on improving understanding of the mechanisms of adaptive immunity to cholera.
3 specific aims of the project are: (1) to assess whether TcpA-specific immune responses are associated with protection from cholera; (2) to assess whether the Peru-15 live attenuated cholera vaccine induces comparable immune responses to natural cholera infection; and (3) to develop a transcutaneous vaccination strategy to boost TcpA-specific immune responses in an animal model of cholera. This research plan will allow the trainee to build on preliminary findings and to develop research skills related to vaccine development that can be used to support an independent research career. Relevance: This research will increase our understanding of immunity to cholera and may aid in the development of a cholera vaccine that could save thousands of lives each year in developing countries.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Research Scientist Development Award - Research & Training (K01)
Project #
5K01TW007409-03
Application #
7265252
Study Section
Special Emphasis Panel (ZRG1-ICP-2 (51))
Program Officer
Jessup, Christine
Project Start
2005-09-16
Project End
2009-08-31
Budget Start
2007-09-01
Budget End
2008-08-31
Support Year
3
Fiscal Year
2007
Total Cost
$112,651
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Falkard, Brie; Uddin, Taher; Rahman, M Arifur et al. (2015) Plasma Leptin Levels in Children Hospitalized with Cholera in Bangladesh. Am J Trop Med Hyg 93:244-9
Uddin, Taher; Aktar, Amena; Xu, Peng et al. (2014) Immune responses to O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 Ogawa in adult Bangladeshi recipients of an oral killed cholera vaccine and comparison to responses in patients with cholera. Am J Trop Med Hyg 90:873-81
Alam, Mohammad Murshid; Bufano, Megan Kelly; Xu, Peng et al. (2014) Evaluation in mice of a conjugate vaccine for cholera made from Vibrio cholerae O1 (Ogawa) O-specific polysaccharide. PLoS Negl Trop Dis 8:e2683
Bhuiyan, Saruar; Sayeed, Abu; Khanam, Farhana et al. (2014) Cellular and cytokine responses to Salmonella enterica serotype Typhi proteins in patients with typhoid fever in Bangladesh. Am J Trop Med Hyg 90:1024-30
Leung, Daniel T; Uddin, Taher; Xu, Peng et al. (2013) Immune responses to the O-specific polysaccharide antigen in children who received a killed oral cholera vaccine compared to responses following natural cholera infection in Bangladesh. Clin Vaccine Immunol 20:780-8
Rahman, Atiqur; Rashu, Rasheduzzaman; Bhuiyan, Taufiqur Rahman et al. (2013) Antibody-secreting cell responses after Vibrio cholerae O1 infection and oral cholera vaccination in adults in Bangladesh. Clin Vaccine Immunol 20:1592-8
Charles, Richelle C; Sultana, Tania; Alam, Mohammad Murshid et al. (2013) Identification of immunogenic Salmonella enterica serotype Typhi antigens expressed in chronic biliary carriers of S. Typhi in Kathmandu, Nepal. PLoS Negl Trop Dis 7:e2335
Alam, Mohammad Murshid; Tsai, Lillian L; Rollins, Sean M et al. (2013) Identification of in vivo-induced bacterial proteins during human infection with Salmonella enterica serotype Paratyphi A. Clin Vaccine Immunol 20:712-9
Alam, Mohammad Murshid; Arifuzzaman, Mohammad; Ahmad, Shaikh Meshbahuddin et al. (2013) Study of avidity of antigen-specific antibody as a means of understanding development of long-term immunological memory after Vibrio cholerae O1 infection. Clin Vaccine Immunol 20:17-23
Karlsson, Elinor K; Harris, Jason B; Tabrizi, Shervin et al. (2013) Natural selection in a bangladeshi population from the cholera-endemic ganges river delta. Sci Transl Med 5:192ra86

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