The immediate goals are to delineate the mechanisms by which functionally important molecules such as Lyb2, Ia and BSF-1 receptors participate in B lymphocyte activation. Long term goals are to devise ways to regulate B cell function in hyper- and hypo-immune states as well as to define the biochemical basis of B cell growth and differentiation using the approaches of biochemistry, cellular immunology and molecular biology. The long term objectives of this research are to understand the underlying basis for the immune abnormalities associated with age. Initially, the focus of the research will be the functional role of two B cell surface molecules Lyb2 and Ia. Strategies will be developed to clone the gene for Lyb2 antigen. The concept that a unique B cell subset is involved in antibody responses to polysaccharide antigens will be evaluated. The possibility that B cell subsets may differ in the growth or maturation factor receptors they express will be studied by preparing monoclonal antibodies to such B cell surface molecules. Biochemical and phenotypic abnormalities in B cells from aged mice will be studied. The repertoire differences in B cells from young and aged mice will be determined by quantitating the expression of the murine heavy chain variable region gene families by nucleic acid hybridization techniques. These studies should enable us to better understand B cell growth and differentiation and to develop better strategies for immunomodulation.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Modified Research Career Development Award (K04)
Project #
5K04AG000422-02
Application #
3070559
Study Section
Immunobiology Study Section (IMB)
Project Start
1988-08-01
Project End
1993-07-01
Budget Start
1989-08-01
Budget End
1990-07-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Kentucky
Department
Type
Schools of Medicine
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506
Sublette, M Elizabeth; Bosetti, Francesca; DeMar, James C et al. (2007) Plasma free polyunsaturated fatty acid levels are associated with symptom severity in acute mania. Bipolar Disord 9:759-65
Muthukkumar, S; Ramesh, T M; Bondada, S (1995) Rapamycin, a potent immunosuppressive drug, causes programmed cell death in B lymphoma cells. Transplantation 60:264-70
Muthukkumar, S; Bondada, S (1995) Involvement of CD5 in Th1 and Th2 contact-mediated rescue of anti-mu and ionomycin induced growth inhibition in a B cell lymphoma. Int Immunol 7:305-15
Garg, M; Kaplan, A M; Bondada, S (1994) Cellular basis of differential responsiveness of lymph nodes and spleen to 23-valent Pnu-Imune vaccine. J Immunol 152:1589-96
Muthukkumar, S; Udhayakumar, V; Bondada, S (1993) Elevation of cytosolic calcium is sufficient to induce growth inhibition in a B cell lymphoma. Eur J Immunol 23:2419-26
Garg, M; Bondada, S (1993) Reversal of age-associated decline in immune response to Pnu-imune vaccine by supplementation with the steroid hormone dehydroepiandrosterone. Infect Immun 61:2238-41
Goud, S N; Kaplan, A M; Subbarao, B (1992) Effects of cigarette smoke on the antibody responses to thymic independent antigens from different lymphoid tissues of mice. Arch Toxicol 66:164-9
Goud, S N; Kaplan, A M; Subbarao, B (1992) Priming of peripheral lymph node B cells with TNP-Ficoll: role of lymphokines in B cell differentiation. Cell Immunol 144:407-16
Garg, M; Subbarao, B (1992) Immune responses of systemic and mucosal lymphoid organs to Pnu-Imune vaccine as a function of age and the efficacy of monophosphoryl lipid A as an adjuvant. Infect Immun 60:2329-36
Udhayakumar, V; Goud, S N; Cross, R J et al. (1991) Physiology of murine B lymphocytes. II. Life-spans of mitogen and thymus-independent antigen (type 2) reactive B lymphocytes from aged mice. Mech Ageing Dev 61:135-47

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