Positron emission tomography (PET) provides non-invasive, quantitative, in vivo, measurements of regional cerebral blood flow (rCBF) and regional cerebral metabolic rate (rCMR) in man. Regional CBF and CMR vary directly with the regional rate of cerebral neuronal activity in the brain. Oxygen 15-H2O PET is a new PET methodology employing oxygen 15 in water (oxygen 15 half life = 123 sec) as a diffusible blood flow tracer to measure rCBF. Oxygen 15-H2O PET is uniquely suited to the study of focal cerebral function because of the brief (40 sec) scan duration, good resolution and the capacity for rapidly sequential scans (8 rCBF scans in 90 min) in a single individual. We propose to use oxygen 15-H2O PET to measure rCBF in normal volunteers during maneuvers designed to induce focal activations of discrete, cortical neuron populations. Initial studies will delineate response characteristics of somatic sensory neurons to electrocutaneous stimuli ranging in frequency from slow (2-10 Hz) to flutter (40-80 Hz) to vibration (100-500 Hz). Stimulus frequencies are chosen on the basis of the known response characteristics of cortical somatic sensory neurons in primates. When stimuli inducing consistent cortical rCBF responses are defined, they will be employed for more complex stimulus paradigms. Complex paradigms will be intended to induce focal cortical changes via cognitive tasks including pattern recognition, directed attention and language, all using somatic sensory stimuli as the vehicle. In this way the activation induced by the simple stimuli themselves will be well known, allowing discrimination of the regional changes due to the higher-order cognitive activity from rCBF change due to the stimulus alone. These experiments will provide a foundation for continued work on human cortical neurophysiology with the long-range intent of exploring progressively more complex human behaviors. Precise quantitation of the cortical CBF changes induced by simple, reproducible stimuli will also be the basis for studies of neurological pathophysiology, as these stimuli may then be used to generate cortical activation in individuals with neurological disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Academic/Teacher Award (ATA) (K07)
Project #
5K07NS000904-03
Application #
3078279
Study Section
Neurological Disorders Program Project Review A Committee (NSPA)
Project Start
1984-07-01
Project End
1989-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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Condic, M L; Bentley, D (1989) Pioneer growth cone adhesion in vivo to boundary cells and neurons after enzymatic removal of basal lamina in grasshopper embryos. J Neurosci 9:2687-96
Mintun, M A; Fox, P T; Raichle, M E (1989) A highly accurate method of localizing regions of neuronal activation in the human brain with positron emission tomography. J Cereb Blood Flow Metab 9:96-103
Reiman, E M; Raichle, M E; Robins, E et al. (1989) Neuroanatomical correlates of a lactate-induced anxiety attack. Arch Gen Psychiatry 46:493-500
Fox, P T; Mintun, M A (1989) Noninvasive functional brain mapping by change-distribution analysis of averaged PET images of H215O tissue activity. J Nucl Med 30:141-9
Fox, P T; Mintun, M A; Reiman, E M et al. (1988) Enhanced detection of focal brain responses using intersubject averaging and change-distribution analysis of subtracted PET images. J Cereb Blood Flow Metab 8:642-53