Two groups of transgenic mice harboring the oncogene (T antigen) from SV40 virus have been produced which display distinct patterns of neuropathology. One group develops tumors in the choroid plexus; the second group develops a demyelinating/hypomyelinating peripheral neuropathy. The proposed study will characterize the pathology which develops in these transgenic mice in terms of the expression of the oncogene which was introduced. The developmental time courses of the choroid plexus tumors and the peripheral neuropathy will be studied morphologically and correlated with the temporal pattern of T antigen gene expression in various tissues. The peripheral neuropathy will be characterized by quantitative morphology. T antigen gene expression will be studied by in situ hybridization using cDNA probes to detect T antigen mRNA, and immunohistochemical localization of T antigen protein using polyclonal and monoclonal antibodies. Whether axons and/or Schwann cells are the primary defect in the peripheral neuropathy will be studied in vivo by transplantation of nerve grafts, and in vitro by recombinations of purified populations of neurons and Schwann cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Academic/Teacher Award (ATA) (K07)
Project #
1K07NS000956-01
Application #
3078369
Study Section
Neurological Disorders Program Project Review A Committee (NSPA)
Project Start
1985-04-01
Project End
1990-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Schools of Veterinary Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715