The candidate is particularly interested in understanding the mechanism by which Hiv evades immune surviellance. An increased understanding of how this occurs may be critical for the development chemotherapeutics and vaccines. In the proposed research, cytotxic t lymphocytes (cTLs) isolated from Hiv postive patients will be tested for their ability tokill primary CD4 postive cell isolates infected with HIV. Career Devleopment Plan: Dr. collins will have no specific training activityes aside from the performance of research. She will participate in weekly laboratory meetings encompassing a broad range of research activities, as well as journal club and periodic scientific meetings. She will be encouraged to attend scientific seminars presented at MIT and surrounding institutes. Research Plan: the research plan takes a phased approach, starting with Hiv-1 infected t-cell lines and cloned DC8 cells. Then primary cells will be infected, purified, and used as targets. CD8 positive cells from Hiv-1 positive individuals will then be substituted for the CD8 clones. Finally, endogenous viruses from HIV-1 positive individuals will then be substituted for the CD8 clones. Finally, endogenous viruses from HIV-1 postive individuals will be engineered to express a selectable marker so that infected cells can be purified.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08AI001448-03
Application #
2886048
Study Section
Special Emphasis Panel (ZAI1-PSS-A (J1))
Program Officer
Sager, Polly R
Project Start
1997-09-01
Project End
2001-08-31
Budget Start
1999-09-01
Budget End
2001-08-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Stohl, Elizabeth A; Dale, Erin M; Criss, Alison K et al. (2013) Neisseria gonorrhoeae metalloprotease NGO1686 is required for full piliation, and piliation is required for resistance to H2O2- and neutrophil-mediated killing. MBio 4:
Stohl, Elizabeth A; Chan, Yolande A; Hackett, Kathleen T et al. (2012) Neisseria gonorrhoeae virulence factor NG1686 is a bifunctional M23B family metallopeptidase that influences resistance to hydrogen peroxide and colony morphology. J Biol Chem 287:11222-33
Stohl, Elizabeth A; Gruenig, Marielle C; Cox, Michael M et al. (2011) Purification and characterization of the RecA protein from Neisseria gonorrhoeae. PLoS One 6:e17101
Bobbitt, Kevin R; Addo, Marylyn M; Altfeld, Marcus et al. (2003) Rev activity determines sensitivity of HIV-1-infected primary T cells to CTL killing. Immunity 18:289-99
Fleis, Rebekah; Filzen, Tracey; Collins, Kathleen L (2002) Species-specific effects of HIV-1 Nef-mediated MHC-I downmodulation. Virology 303:120-9
Swann, S A; Williams, M; Story, C M et al. (2001) HIV-1 Nef blocks transport of MHC class I molecules to the cell surface via a PI 3-kinase-dependent pathway. Virology 282:267-77
Collins, K L; Baltimore, D (1999) HIV's evasion of the cellular immune response. Immunol Rev 168:65-74