The repertoire of glycoproteins expressed on the plasma membrane of lymphoid cells reflects their state of differentiation and maturation. Many of these molecules were first identified using monoclonal antibodies raised against neoplastic cells. In the case of non T, non B acute lymphoblastic leukemia cells, several membrane antigens have been identified whose expression on lymphocytes is limited to the early B lineage. The physiologic function of these gene products is unknown. Four of these antigens are represented in single polypeptides and thus appear to be encoded by single genes. The low level of expression of these gene products suggests that they are encoded by RNA transcripts which are present in low abundance. We, therefore, propose to use DNA-mediated gene transfer to express the genes encoding human B lymphocyte differentiation antigens in mouse cell lines. The donor human DNA sequences will be isolated by serial DNA transfection and will be molecularly cloned. Restriction fragments lacking highly repetitive human DNA sequences will be identified and are expected to contain the coding sequences (exons) expressed in spliced, polyadenylated messenger RNAs. The detection of such sequences in cDNA libraries provides an alternative approach to cloning rare transcripts and determining their nucleotide sequence. The first detailed characterization of genes encoding proteins whose expression in clonal hematopoietic malignancies is of diagnostic significance will provide the necessary tools to evaluate the expression of these genes in normal B cell development.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08CA001013-03
Application #
3079475
Study Section
(SRC)
Project Start
1985-05-01
Project End
1988-04-30
Budget Start
1987-05-01
Budget End
1988-04-30
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
St. Jude Children's Research Hospital
Department
Type
DUNS #
067717892
City
Memphis
State
TN
Country
United States
Zip Code
38105
Sanoff, Hanna K; Carpenter, William R; Sturmer, Til et al. (2012) Effect of adjuvant chemotherapy on survival of patients with stage III colon cancer diagnosed after age 75 years. J Clin Oncol 30:2624-34
Rogers Jr, Selwyn O; Gray, Stacy W; Landrum, Mary Beth et al. (2010) Variations in surgeon treatment recommendations for lobectomy in early-stage non-small-cell lung cancer by patient age and comorbidity. Ann Surg Oncol 17:1581-8
Keating, Nancy L; Beth Landrum, Mary; Arora, Neeraj K et al. (2010) Cancer patients' roles in treatment decisions: do characteristics of the decision influence roles? J Clin Oncol 28:4364-70
Chrischilles, Elizabeth A; Pendergast, Jane F; Kahn, Katherine L et al. (2010) Adverse events among the elderly receiving chemotherapy for advanced non-small-cell lung cancer. J Clin Oncol 28:620-7
Kahn, Katherine L; Adams, John L; Weeks, Jane C et al. (2010) Adjuvant chemotherapy use and adverse events among older patients with stage III colon cancer. JAMA 303:1037-45
Keating, Nancy L; Landrum, Mary Beth; Rogers Jr, Selwyn O et al. (2010) Physician factors associated with discussions about end-of-life care. Cancer 116:998-1006
Earle, Craig C; Chretien, Yves; Morris, Carl et al. (2010) Employment among survivors of lung cancer and colorectal cancer. J Clin Oncol 28:1700-5
Klabunde, Carrie N; Ambs, Anita; Keating, Nancy L et al. (2009) The role of primary care physicians in cancer care. J Gen Intern Med 24:1029-36
Rogers Jr, Selwyn O; Ayanian, John Z; Ko, Clifford Y et al. (2009) Surgeons' volume of colorectal cancer procedures and collaborative decision-making about adjuvant therapies. Ann Surg 250:895-900
Zafar, S Yousuf; Abernethy, Amy P; Abbott, David H et al. (2008) Comorbidity, age, race and stage at diagnosis in colorectal cancer: a retrospective, parallel analysis of two health systems. BMC Cancer 8:345

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